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pd l1 r30949 antibody  (NSJ Bioreagents)


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    NSJ Bioreagents pd l1 r30949 antibody
    UA regulated EGFR/JAK2/STAT3 signaling in NSCLC cells. ( A ) Molecular docking using AutoDock Vina software showed UA binding (PubChem ID: 64945) to the ATP-binding domain of EGFR (PDB ID: 2GS2). ( B ) Western blotting of the lysate of the A549 and H460 cells pre-treated with recombinant EGF (25 ng/mL) for 1 h and then with 20 µM UA for 24 h, showing the levels of phosphorylated EGFR and total EGFR. The representative levels of the proteins were determined by densitometry and normalized to β-actin, which was set to 100. The data were confirmed after repeating the experiment 3 times. ( C ) Western blotting of the proteins involved in EGFR/JAK2/STAT3 signaling and <t>PD-L1</t> in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-Actin was used as the housekeeping protein. ( D ) Western blotting for MMPs and VEGF in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-actin used as a housekeeping protein. (E) RT-qPCR analysis for the expression of MMP2 , MMP3 , MMP9 , VEGF , and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. The representative expressions of MMP2 , MMP3 , MMP9 , VEGF and PD-L1 were shown. The Cp values were normalized to that of GAPDH , which was set to 100. *** p < 0.001 (ANOVA test) and # p < 0.001 vs. control.
    Pd L1 R30949 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pd l1 r30949 antibody/product/NSJ Bioreagents
    Average 90 stars, based on 2 article reviews
    pd l1 r30949 antibody - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer Cells"

    Article Title: Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer Cells

    Journal: Biomedicines

    doi: 10.3390/biomedicines9030297

    UA regulated EGFR/JAK2/STAT3 signaling in NSCLC cells. ( A ) Molecular docking using AutoDock Vina software showed UA binding (PubChem ID: 64945) to the ATP-binding domain of EGFR (PDB ID: 2GS2). ( B ) Western blotting of the lysate of the A549 and H460 cells pre-treated with recombinant EGF (25 ng/mL) for 1 h and then with 20 µM UA for 24 h, showing the levels of phosphorylated EGFR and total EGFR. The representative levels of the proteins were determined by densitometry and normalized to β-actin, which was set to 100. The data were confirmed after repeating the experiment 3 times. ( C ) Western blotting of the proteins involved in EGFR/JAK2/STAT3 signaling and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-Actin was used as the housekeeping protein. ( D ) Western blotting for MMPs and VEGF in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-actin used as a housekeeping protein. (E) RT-qPCR analysis for the expression of MMP2 , MMP3 , MMP9 , VEGF , and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. The representative expressions of MMP2 , MMP3 , MMP9 , VEGF and PD-L1 were shown. The Cp values were normalized to that of GAPDH , which was set to 100. *** p < 0.001 (ANOVA test) and # p < 0.001 vs. control.
    Figure Legend Snippet: UA regulated EGFR/JAK2/STAT3 signaling in NSCLC cells. ( A ) Molecular docking using AutoDock Vina software showed UA binding (PubChem ID: 64945) to the ATP-binding domain of EGFR (PDB ID: 2GS2). ( B ) Western blotting of the lysate of the A549 and H460 cells pre-treated with recombinant EGF (25 ng/mL) for 1 h and then with 20 µM UA for 24 h, showing the levels of phosphorylated EGFR and total EGFR. The representative levels of the proteins were determined by densitometry and normalized to β-actin, which was set to 100. The data were confirmed after repeating the experiment 3 times. ( C ) Western blotting of the proteins involved in EGFR/JAK2/STAT3 signaling and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-Actin was used as the housekeeping protein. ( D ) Western blotting for MMPs and VEGF in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-actin used as a housekeeping protein. (E) RT-qPCR analysis for the expression of MMP2 , MMP3 , MMP9 , VEGF , and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. The representative expressions of MMP2 , MMP3 , MMP9 , VEGF and PD-L1 were shown. The Cp values were normalized to that of GAPDH , which was set to 100. *** p < 0.001 (ANOVA test) and # p < 0.001 vs. control.

    Techniques Used: Software, Binding Assay, Western Blot, Recombinant, Quantitative RT-PCR, Expressing

    UA inhibited the binding of STAT3 to the MMP2 and PD-L1 promoters. ( A ) ChIP assay showed that treatment with 20 µM UA inhibited the formation of the STAT3/MMP2 and STAT3/PD-L1 complexes in A549 and H460 cells. The relative binding of STAT3 to the MMP2 and PD-L1 promoters was expressed as a percentage of the control. Statistical analysis was performed using Student’s t -test (** p < 0.01). ( B ) Western blotting of A549 and H460 cells treated with 30 pM STAT3 siRNA or 20 µM UA for 24 h showing the patterns of phosphorylated STAT3, STAT3, PD-L1, and MMP2 protein levels. β-actin was used as a housekeeping protein.
    Figure Legend Snippet: UA inhibited the binding of STAT3 to the MMP2 and PD-L1 promoters. ( A ) ChIP assay showed that treatment with 20 µM UA inhibited the formation of the STAT3/MMP2 and STAT3/PD-L1 complexes in A549 and H460 cells. The relative binding of STAT3 to the MMP2 and PD-L1 promoters was expressed as a percentage of the control. Statistical analysis was performed using Student’s t -test (** p < 0.01). ( B ) Western blotting of A549 and H460 cells treated with 30 pM STAT3 siRNA or 20 µM UA for 24 h showing the patterns of phosphorylated STAT3, STAT3, PD-L1, and MMP2 protein levels. β-actin was used as a housekeeping protein.

    Techniques Used: Binding Assay, Western Blot

    The molecular regulatory mechanism underlying UA’s anticancer activity in NSCLC cells, and UA’s inhibition of the EGFR/JAK2/STAT3 signaling pathway and PD-L1. Continuous black point arrow denotes the activity of UA, dotted point arrows denotes blockage of signals, and red block arrow denotes the inhibition of EGFR signaling by UA.
    Figure Legend Snippet: The molecular regulatory mechanism underlying UA’s anticancer activity in NSCLC cells, and UA’s inhibition of the EGFR/JAK2/STAT3 signaling pathway and PD-L1. Continuous black point arrow denotes the activity of UA, dotted point arrows denotes blockage of signals, and red block arrow denotes the inhibition of EGFR signaling by UA.

    Techniques Used: Activity Assay, Inhibition, Blocking Assay



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    pd l1 r30949 antibody  (NSJ Bioreagents)
    90
    NSJ Bioreagents pd l1 r30949 antibody
    UA regulated EGFR/JAK2/STAT3 signaling in NSCLC cells. ( A ) Molecular docking using AutoDock Vina software showed UA binding (PubChem ID: 64945) to the ATP-binding domain of EGFR (PDB ID: 2GS2). ( B ) Western blotting of the lysate of the A549 and H460 cells pre-treated with recombinant EGF (25 ng/mL) for 1 h and then with 20 µM UA for 24 h, showing the levels of phosphorylated EGFR and total EGFR. The representative levels of the proteins were determined by densitometry and normalized to β-actin, which was set to 100. The data were confirmed after repeating the experiment 3 times. ( C ) Western blotting of the proteins involved in EGFR/JAK2/STAT3 signaling and <t>PD-L1</t> in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-Actin was used as the housekeeping protein. ( D ) Western blotting for MMPs and VEGF in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-actin used as a housekeeping protein. (E) RT-qPCR analysis for the expression of MMP2 , MMP3 , MMP9 , VEGF , and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. The representative expressions of MMP2 , MMP3 , MMP9 , VEGF and PD-L1 were shown. The Cp values were normalized to that of GAPDH , which was set to 100. *** p < 0.001 (ANOVA test) and # p < 0.001 vs. control.
    Pd L1 R30949 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pd l1 r30949 antibody/product/NSJ Bioreagents
    Average 90 stars, based on 1 article reviews
    pd l1 r30949 antibody - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    UA regulated EGFR/JAK2/STAT3 signaling in NSCLC cells. ( A ) Molecular docking using AutoDock Vina software showed UA binding (PubChem ID: 64945) to the ATP-binding domain of EGFR (PDB ID: 2GS2). ( B ) Western blotting of the lysate of the A549 and H460 cells pre-treated with recombinant EGF (25 ng/mL) for 1 h and then with 20 µM UA for 24 h, showing the levels of phosphorylated EGFR and total EGFR. The representative levels of the proteins were determined by densitometry and normalized to β-actin, which was set to 100. The data were confirmed after repeating the experiment 3 times. ( C ) Western blotting of the proteins involved in EGFR/JAK2/STAT3 signaling and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-Actin was used as the housekeeping protein. ( D ) Western blotting for MMPs and VEGF in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-actin used as a housekeeping protein. (E) RT-qPCR analysis for the expression of MMP2 , MMP3 , MMP9 , VEGF , and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. The representative expressions of MMP2 , MMP3 , MMP9 , VEGF and PD-L1 were shown. The Cp values were normalized to that of GAPDH , which was set to 100. *** p < 0.001 (ANOVA test) and # p < 0.001 vs. control.

    Journal: Biomedicines

    Article Title: Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer Cells

    doi: 10.3390/biomedicines9030297

    Figure Lengend Snippet: UA regulated EGFR/JAK2/STAT3 signaling in NSCLC cells. ( A ) Molecular docking using AutoDock Vina software showed UA binding (PubChem ID: 64945) to the ATP-binding domain of EGFR (PDB ID: 2GS2). ( B ) Western blotting of the lysate of the A549 and H460 cells pre-treated with recombinant EGF (25 ng/mL) for 1 h and then with 20 µM UA for 24 h, showing the levels of phosphorylated EGFR and total EGFR. The representative levels of the proteins were determined by densitometry and normalized to β-actin, which was set to 100. The data were confirmed after repeating the experiment 3 times. ( C ) Western blotting of the proteins involved in EGFR/JAK2/STAT3 signaling and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-Actin was used as the housekeeping protein. ( D ) Western blotting for MMPs and VEGF in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-actin used as a housekeeping protein. (E) RT-qPCR analysis for the expression of MMP2 , MMP3 , MMP9 , VEGF , and PD-L1 in the A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. The representative expressions of MMP2 , MMP3 , MMP9 , VEGF and PD-L1 were shown. The Cp values were normalized to that of GAPDH , which was set to 100. *** p < 0.001 (ANOVA test) and # p < 0.001 vs. control.

    Article Snippet: The Cyclin D1 (ab6152) antibody (Abcam, Cambridge, MA, USA), MMP2 (E90317) antibody (EnoGene, New York, NY, USA), and the PD-L1 (R30949) antibody (NSJ Bioreagents, San Diego, CA, USA) were procured.

    Techniques: Software, Binding Assay, Western Blot, Recombinant, Quantitative RT-PCR, Expressing

    UA inhibited the binding of STAT3 to the MMP2 and PD-L1 promoters. ( A ) ChIP assay showed that treatment with 20 µM UA inhibited the formation of the STAT3/MMP2 and STAT3/PD-L1 complexes in A549 and H460 cells. The relative binding of STAT3 to the MMP2 and PD-L1 promoters was expressed as a percentage of the control. Statistical analysis was performed using Student’s t -test (** p < 0.01). ( B ) Western blotting of A549 and H460 cells treated with 30 pM STAT3 siRNA or 20 µM UA for 24 h showing the patterns of phosphorylated STAT3, STAT3, PD-L1, and MMP2 protein levels. β-actin was used as a housekeeping protein.

    Journal: Biomedicines

    Article Title: Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer Cells

    doi: 10.3390/biomedicines9030297

    Figure Lengend Snippet: UA inhibited the binding of STAT3 to the MMP2 and PD-L1 promoters. ( A ) ChIP assay showed that treatment with 20 µM UA inhibited the formation of the STAT3/MMP2 and STAT3/PD-L1 complexes in A549 and H460 cells. The relative binding of STAT3 to the MMP2 and PD-L1 promoters was expressed as a percentage of the control. Statistical analysis was performed using Student’s t -test (** p < 0.01). ( B ) Western blotting of A549 and H460 cells treated with 30 pM STAT3 siRNA or 20 µM UA for 24 h showing the patterns of phosphorylated STAT3, STAT3, PD-L1, and MMP2 protein levels. β-actin was used as a housekeeping protein.

    Article Snippet: The Cyclin D1 (ab6152) antibody (Abcam, Cambridge, MA, USA), MMP2 (E90317) antibody (EnoGene, New York, NY, USA), and the PD-L1 (R30949) antibody (NSJ Bioreagents, San Diego, CA, USA) were procured.

    Techniques: Binding Assay, Western Blot

    The molecular regulatory mechanism underlying UA’s anticancer activity in NSCLC cells, and UA’s inhibition of the EGFR/JAK2/STAT3 signaling pathway and PD-L1. Continuous black point arrow denotes the activity of UA, dotted point arrows denotes blockage of signals, and red block arrow denotes the inhibition of EGFR signaling by UA.

    Journal: Biomedicines

    Article Title: Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer Cells

    doi: 10.3390/biomedicines9030297

    Figure Lengend Snippet: The molecular regulatory mechanism underlying UA’s anticancer activity in NSCLC cells, and UA’s inhibition of the EGFR/JAK2/STAT3 signaling pathway and PD-L1. Continuous black point arrow denotes the activity of UA, dotted point arrows denotes blockage of signals, and red block arrow denotes the inhibition of EGFR signaling by UA.

    Article Snippet: The Cyclin D1 (ab6152) antibody (Abcam, Cambridge, MA, USA), MMP2 (E90317) antibody (EnoGene, New York, NY, USA), and the PD-L1 (R30949) antibody (NSJ Bioreagents, San Diego, CA, USA) were procured.

    Techniques: Activity Assay, Inhibition, Blocking Assay